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mmp3 (R&D Systems)

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R&D Systems mmp3
The VCA-D4. A) Schematic representation of the VCA-D4 with its three-zone architecture. Zone 1 contains the TAC-D4 competitive assay format while Zones 2 and 3 have a traditional sandwich immunoassay format for <t>MMP3</t> and IL-6 respectfully. B) VCA-D4 dose response curves for tacrolimus (gray), MMP3 (yellow) and IL-6 (blue) using fetal bovine serum.

The VCA-D4. A) Schematic representation of the VCA-D4 with its three-zone architecture. Zone 1 contains the TAC-D4 competitive assay format while Zones 2 and 3 have a traditional sandwich immunoassay format for <t>MMP3</t> and IL-6 respectfully. B) VCA-D4 dose response curves for tacrolimus (gray), MMP3 (yellow) and IL-6 (blue) using fetal bovine serum.

Mmp3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dmp300/Human+Total+MMP-3+Quantikine+ELISA+Kit/pmc13614467-101-20-21
Average 93 stars, based on 108 article reviews

mmp3 - by Bioz Stars, 2026-10

93/100 stars

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1) Product Images from "A point-of-care test to measure tacrolimus directly from unprocessed whole blood"

Article Title: A point-of-care test to measure tacrolimus directly from unprocessed whole blood

Journal: Analytical chemistry

doi: 10.1021/acs.analchem.5c07803

Figure Legend Snippet: The VCA-D4. A) Schematic representation of the VCA-D4 with its three-zone architecture. Zone 1 contains the TAC-D4 competitive assay format while Zones 2 and 3 have a traditional sandwich immunoassay format for MMP3 and IL-6 respectfully. B) VCA-D4 dose response curves for tacrolimus (gray), MMP3 (yellow) and IL-6 (blue) using fetal bovine serum.

Techniques Used:

Comparison of the performance of the VCA-D4 to commercial ELISAs. A) Correlation plot between the fluorescence response of the VCA-D4 and the measured absorbance from the R&D Systems ® IL-6 ELISA using the ELISA IL-6 standard (R 2 =0.95). B) Correlation plot between the fluorescence response of the VCA-D4 and the measured absorbance from the R&D Systems ® IL-6 ELISA (R 2 =0.97) using the selected IL-6 protein used for VCA-D4 development. C) Correlation plot between the fluorescence response of the VCA-D4 and the measured absorbance from the R&D Systems ® MMP3 ELISA using the ELISA MMP3 standard (R 2 =0.71 when using all points and 0.82 when using points above 0.1 ng/mL–the LOD of the VCA-D4). D) Correlation plot between the fluorescence response of the VCA-D4 and the measured absorbance from the R&D Systems ® MMP3 ELISA (R 2 =0.7 for all points, 0.8 for points above 1 ng/mL) using the selected MMP3 protein used for VCA-D4 development.

Figure Legend Snippet: Comparison of the performance of the VCA-D4 to commercial ELISAs. A) Correlation plot between the fluorescence response of the VCA-D4 and the measured absorbance from the R&D Systems ® IL-6 ELISA using the ELISA IL-6 standard (R 2 =0.95). B) Correlation plot between the fluorescence response of the VCA-D4 and the measured absorbance from the R&D Systems ® IL-6 ELISA (R 2 =0.97) using the selected IL-6 protein used for VCA-D4 development. C) Correlation plot between the fluorescence response of the VCA-D4 and the measured absorbance from the R&D Systems ® MMP3 ELISA using the ELISA MMP3 standard (R 2 =0.71 when using all points and 0.82 when using points above 0.1 ng/mL–the LOD of the VCA-D4). D) Correlation plot between the fluorescence response of the VCA-D4 and the measured absorbance from the R&D Systems ® MMP3 ELISA (R 2 =0.7 for all points, 0.8 for points above 1 ng/mL) using the selected MMP3 protein used for VCA-D4 development.

Techniques Used: Comparison, Fluorescence, Enzyme-linked Immunosorbent Assay

other:

Article Title: Evaluation of Chemokines in the Gingival Crevicular Fluid of Children with Down Syndrome
Article Snippet: DMP300 and DTM100; R and D Systems).

Article Title: Polymorphism in the matrix metallopeptidase genes is associated with coronary artery disease risk with concomitant metabolic syndrome
Article Snippet: DMP300, R&D Systems, USA), MMP-9 (кат.

Enzyme-linked Immunosorbent Assay:

Article Title: MMP3 Is a Non-invasive Biomarker of Rejection in Skin-Bearing Vascularized Composite Allotransplantation: A Multicenter Validation Study
Article Snippet: .. Serum concentrations of MMP3 were measured by ELISA, using a commercially available kit (R&D Systems cat.# DMP300, Minneapolis, MN), according to the manufacturer's instructions, as previously described ( ). ..

Clinical Proteomics:

Article Title: Alzheimer’s disease with sleep insufficiency: a cross-sectional study on correlations among clinical characteristics, orexin, its receptors, and the blood-brain barrier
Article Snippet: .. MMP200, DMP300, DMP900, and DRG00 kits (R&D Systems Company, Minneapolis, MN, USA) were used to evaluate the levels of MMP-2, MMP-3, MMP-9 and RAGE in the CSF, respectively. .. An MBS772326 kit (MyBioSource Company, San Diego, CA, USA) was used for LRP1 and a NS830 kit (Merck Millipore Company, Darmstadt, Germany) was used for GFAP.

Image Search Results

Journal: Analytical chemistry

Article Title: A point-of-care test to measure tacrolimus directly from unprocessed whole blood

doi: 10.1021/acs.analchem.5c07803

Figure Lengend Snippet: The VCA-D4. A) Schematic representation of the VCA-D4 with its three-zone architecture. Zone 1 contains the TAC-D4 competitive assay format while Zones 2 and 3 have a traditional sandwich immunoassay format for MMP3 and IL-6 respectfully. B) VCA-D4 dose response curves for tacrolimus (gray), MMP3 (yellow) and IL-6 (blue) using fetal bovine serum.

Article Snippet: For ELISA comparison studies, R&D Systems Quantikine ELISA kits were used for both IL-6 (R&D Systems Catalog Number: D6050) and MMP3 (R&D Systems Catalog Number: DMP300).

Techniques:

Journal: Analytical chemistry

Article Title: A point-of-care test to measure tacrolimus directly from unprocessed whole blood

doi: 10.1021/acs.analchem.5c07803

Figure Lengend Snippet: Comparison of the performance of the VCA-D4 to commercial ELISAs. A) Correlation plot between the fluorescence response of the VCA-D4 and the measured absorbance from the R&D Systems ® IL-6 ELISA using the ELISA IL-6 standard (R 2 =0.95). B) Correlation plot between the fluorescence response of the VCA-D4 and the measured absorbance from the R&D Systems ® IL-6 ELISA (R 2 =0.97) using the selected IL-6 protein used for VCA-D4 development. C) Correlation plot between the fluorescence response of the VCA-D4 and the measured absorbance from the R&D Systems ® MMP3 ELISA using the ELISA MMP3 standard (R 2 =0.71 when using all points and 0.82 when using points above 0.1 ng/mL–the LOD of the VCA-D4). D) Correlation plot between the fluorescence response of the VCA-D4 and the measured absorbance from the R&D Systems ® MMP3 ELISA (R 2 =0.7 for all points, 0.8 for points above 1 ng/mL) using the selected MMP3 protein used for VCA-D4 development.

Article Snippet: For ELISA comparison studies, R&D Systems Quantikine ELISA kits were used for both IL-6 (R&D Systems Catalog Number: D6050) and MMP3 (R&D Systems Catalog Number: DMP300).

Techniques: Comparison, Fluorescence, Enzyme-linked Immunosorbent Assay

( a ) Agarose gel electrophoresis of the WT and c.699+3 A > G mutant NCOA7 transcript. WT, wild-type; Mut, mutation. ( b ) RT‒qPCR results showing the mRNA levels of NCOA7 in KGN cells overexpressing the WT and different mutant NCOA7 vectors (n = 3 biological replicates per group). ****P < 0.0001, ns P > 0.9999, ns P = 0.9996, ns P = 0.6501, ns P = 0.9999, ns P = 0.9996. ( c ) Immunofluorescence images showing the localization of NCOA7 in KGN cells overexpressing the WT and different mutant NCOA7 vectors. Scale bar: 5 μm. ( d ) Quantification of cell proliferation in NCOA7 -KO KGN cells overexpressing the WT or mutant vectors (n = 5 biological replicates per group). ****P < 0.0001, ****P < 0.0001. ( e ) Immunofluorescence images of p21 Cip1 and SA-β-gal in NCOA7 -KO KGN cells overexpressing the WT or mutant vectors. Scale bar: 10 μm. ( f ) RT‒qPCR analysis of expression levels of SASP genes ( IL1B , IL8 , MMP3 , CCL2 ) in NCOA7 -KO KGN cells overexpressing the WT or mutant vectors (n = 4 biological replicates per group). **P = 0.0071, ***P = 0.0001, **P = 0.0033, *P = 0.0152, ns P = 0.0801, **P = 0.0048, ****P < 0.0001, ***P = 0.0004, *P = 0.0245, ***P = 0.0006, ****P < 0.0001, **P = 0.0018. Data in b , d and f are expressed as means ± s.e.m and compared by one-way ANOVA with Dunnett’s post hoc test.

Journal: Nature Aging

Article Title: Stress granule clearance mediated by V-ATPase-interacting protein NCOA7 mitigates ovarian aging

doi: 10.1038/s43587-025-00927-w

Figure Lengend Snippet: ( a ) Agarose gel electrophoresis of the WT and c.699+3 A > G mutant NCOA7 transcript. WT, wild-type; Mut, mutation. ( b ) RT‒qPCR results showing the mRNA levels of NCOA7 in KGN cells overexpressing the WT and different mutant NCOA7 vectors (n = 3 biological replicates per group). ****P < 0.0001, ns P > 0.9999, ns P = 0.9996, ns P = 0.6501, ns P = 0.9999, ns P = 0.9996. ( c ) Immunofluorescence images showing the localization of NCOA7 in KGN cells overexpressing the WT and different mutant NCOA7 vectors. Scale bar: 5 μm. ( d ) Quantification of cell proliferation in NCOA7 -KO KGN cells overexpressing the WT or mutant vectors (n = 5 biological replicates per group). ****P < 0.0001, ****P < 0.0001. ( e ) Immunofluorescence images of p21 Cip1 and SA-β-gal in NCOA7 -KO KGN cells overexpressing the WT or mutant vectors. Scale bar: 10 μm. ( f ) RT‒qPCR analysis of expression levels of SASP genes ( IL1B , IL8 , MMP3 , CCL2 ) in NCOA7 -KO KGN cells overexpressing the WT or mutant vectors (n = 4 biological replicates per group). **P = 0.0071, ***P = 0.0001, **P = 0.0033, *P = 0.0152, ns P = 0.0801, **P = 0.0048, ****P < 0.0001, ***P = 0.0004, *P = 0.0245, ***P = 0.0006, ****P < 0.0001, **P = 0.0018. Data in b , d and f are expressed as means ± s.e.m and compared by one-way ANOVA with Dunnett’s post hoc test.

Article Snippet: The concentrations of IL-1β, IL-8, MMP3 and CCL2 from wild-type, NCOA7 -KO and NCOA7 -rescued KGN cell supernatants were assayed by using the human IL-1 β/IL-1F2 Quantikine ELISA kit (R&D Systems, DLB50), the human IL-8/CXCL8 Quantikine ELISA kit (R&D Systems, D8000C), the human MMP3 Quantikine ELISA kit (R&D Systems, DMP300) and the human CCL2/MCP-1 Quantikine ELISA kit (R&D Systems, DCP00), according to the manufacturers’ protocols.

Techniques: Agarose Gel Electrophoresis, Mutagenesis, Immunofluorescence, Expressing

( a ) Quantification of the apoptosis rate in WT and NCOA7 -KO KGN cells (n = 5 biological replicates per group). ns P = 0.3981. ( b ) Quantification of cell proliferation in WT, NCOA7 -KO, and NCOA7 -rescued KGN cells (n = 5 biological replicates per group). Scale bar: 5 μm. The rescued group was compared with the KO group (#). ####P < 0.0001, ****P < 0.0001, ####P < 0.0001, ****P < 0.0001. ( c ) Quantification of IL-1β, IL-8, MMP3 and CCL2 in the supernatants of WT, NCOA7 -KO, and NCOA7 -rescued KGN cells (n = 5 biological replicates per group). The rescue group was compared with the KO group (#). *P = 0.0172, #P = 0.0206, *P = 0.0115, #P = 0.0279, **P = 0.0030, ##P = 0.0054, **P = 0.0025, #P = 0.0181. ( d ) Western blot of p16 INK4a and p21 Cip1 in WT, NCOA7 -KO, and NCOA7 -rescued KGN cells. ( e ) PCR genotyping of WT and Ncoa7 − / − mice. ( f ) Western blot of ovarian tissues to confirm absence of NCOA7 protein expression in Ncoa7 − / − mice. ( g ) The serum levels of E2 and FSH in 10-month-old WT and Ncoa7 − / − mice (n = 5 biological replicates per group). *P = 0.0224, *P = 0.0412. ( h ) The ovary index in 10-month-old WT and Ncoa7 − / − mice (n = 4 biological replicates per group). ns P = 0.6741. ( i ) Immunohistochemistry analysis of p16 INK4a and p21 Cip1 in ovaries of WT and Ncoa7 − / − mice (n = 3 biological replicates per group). Scale bar: 100 μm. *P = 0.0135, ***P = 0.0006. ( j-k ) Quantification of cell proliferation (n = 3 biological replicates per group) ( j ) and expression levels of SASP genes determined via RT‒qPCR (n = 4 biological replicates per group) ( k ) in GCs from WT and Ncoa7 − / − mice. ***P = 0.0010, ***P = 0.0003 in (r). ns P = 0.0661, **P = 0.0072, *P = 0.0241, *P = 0.0196 in (s). ( l ) Quantification of E2 production in the supernatants of GCs isolated from WT and Ncoa7 − / − mice (n = 4 biological replicates per group). *P = 0.0106. Data in a - c , g - h , and j - l are expressed as means ± s.e.m, and compared by two-tailed Student’s t-test.

Journal: Nature Aging

Article Title: Stress granule clearance mediated by V-ATPase-interacting protein NCOA7 mitigates ovarian aging

doi: 10.1038/s43587-025-00927-w

Figure Lengend Snippet: ( a ) Quantification of the apoptosis rate in WT and NCOA7 -KO KGN cells (n = 5 biological replicates per group). ns P = 0.3981. ( b ) Quantification of cell proliferation in WT, NCOA7 -KO, and NCOA7 -rescued KGN cells (n = 5 biological replicates per group). Scale bar: 5 μm. The rescued group was compared with the KO group (#). ####P < 0.0001, ****P < 0.0001, ####P < 0.0001, ****P < 0.0001. ( c ) Quantification of IL-1β, IL-8, MMP3 and CCL2 in the supernatants of WT, NCOA7 -KO, and NCOA7 -rescued KGN cells (n = 5 biological replicates per group). The rescue group was compared with the KO group (#). *P = 0.0172, #P = 0.0206, *P = 0.0115, #P = 0.0279, **P = 0.0030, ##P = 0.0054, **P = 0.0025, #P = 0.0181. ( d ) Western blot of p16 INK4a and p21 Cip1 in WT, NCOA7 -KO, and NCOA7 -rescued KGN cells. ( e ) PCR genotyping of WT and Ncoa7 − / − mice. ( f ) Western blot of ovarian tissues to confirm absence of NCOA7 protein expression in Ncoa7 − / − mice. ( g ) The serum levels of E2 and FSH in 10-month-old WT and Ncoa7 − / − mice (n = 5 biological replicates per group). *P = 0.0224, *P = 0.0412. ( h ) The ovary index in 10-month-old WT and Ncoa7 − / − mice (n = 4 biological replicates per group). ns P = 0.6741. ( i ) Immunohistochemistry analysis of p16 INK4a and p21 Cip1 in ovaries of WT and Ncoa7 − / − mice (n = 3 biological replicates per group). Scale bar: 100 μm. *P = 0.0135, ***P = 0.0006. ( j-k ) Quantification of cell proliferation (n = 3 biological replicates per group) ( j ) and expression levels of SASP genes determined via RT‒qPCR (n = 4 biological replicates per group) ( k ) in GCs from WT and Ncoa7 − / − mice. ***P = 0.0010, ***P = 0.0003 in (r). ns P = 0.0661, **P = 0.0072, *P = 0.0241, *P = 0.0196 in (s). ( l ) Quantification of E2 production in the supernatants of GCs isolated from WT and Ncoa7 − / − mice (n = 4 biological replicates per group). *P = 0.0106. Data in a - c , g - h , and j - l are expressed as means ± s.e.m, and compared by two-tailed Student’s t-test.

Techniques: Western Blot, Expressing, Immunohistochemistry, Isolation, Two Tailed Test

( a ) Western blot of LC3 and p62 protein levels in WT and NCOA7 -KO KGN cells treated with DMSO or rapamycin. ( b ) SA-β-gal staining of WT, NCOA7 -KO, and NCOA7 -mutant KGN cells. Scale bar: 50 μm. ( c ) Immunofluorescence and quantitative analysis of G3BP1 and NCOA7 in WT and NCOA7 -KO KGN cells (n = 5 biological replicates per group) treated with DMSO or rapamycin. Scale bar: 20 μm. **P = 0.0013, *P = 0.0173. ( d ) Immunofluorescence and quantitative analysis of SGs in ATG7 -knockdown KGN cells (n = 10 views in quantification of stressed cells; n = 20 views per group in quantification of SGs per cell). Scale bar: 15 μm. ****P < 0.0001, ns P = 0.0738; ****P < 0.0001, ns P = 0.8588. ( e ) Quantification of cell proliferation of GCs from 3-NP-treated WT and Ncoa7 − / − mice treated with DMSO or rapamycin (n = 3 biological replicates per group). *P = 0.0351, *P = 0.0131. ( f ) Quantification of expression levels of SASP genes ( Il1b , Il8 , Mmp3 and Ccl2 ) via RT‒qPCR in GCs isolated from 3-NP-treated WT and Ncoa7 − / − mice treated with DMSO or rapamycin (n = 4 biological replicates per group). ns P = 0.8979, ns P = 0.7816, ns P = 0.3738, ns P = 0.6572, *P = 0.0376, *P = 0.0321, **P = 0.0070, **P = 0.0045. ( g ) Quantification of the ovary index in 3-NP-treated WT and Ncoa7 − / − mice treated with DMSO or rapamycin (n = 8 biological replicates per group). ns P = 0.2740, ns P = 0.0695. Data in c - g are expressed as means ± s.e.m, and compared by two-tailed Student’s t-test.

Journal: Nature Aging

Article Title: Stress granule clearance mediated by V-ATPase-interacting protein NCOA7 mitigates ovarian aging

doi: 10.1038/s43587-025-00927-w

Figure Lengend Snippet: ( a ) Western blot of LC3 and p62 protein levels in WT and NCOA7 -KO KGN cells treated with DMSO or rapamycin. ( b ) SA-β-gal staining of WT, NCOA7 -KO, and NCOA7 -mutant KGN cells. Scale bar: 50 μm. ( c ) Immunofluorescence and quantitative analysis of G3BP1 and NCOA7 in WT and NCOA7 -KO KGN cells (n = 5 biological replicates per group) treated with DMSO or rapamycin. Scale bar: 20 μm. **P = 0.0013, *P = 0.0173. ( d ) Immunofluorescence and quantitative analysis of SGs in ATG7 -knockdown KGN cells (n = 10 views in quantification of stressed cells; n = 20 views per group in quantification of SGs per cell). Scale bar: 15 μm. ****P < 0.0001, ns P = 0.0738; ****P < 0.0001, ns P = 0.8588. ( e ) Quantification of cell proliferation of GCs from 3-NP-treated WT and Ncoa7 − / − mice treated with DMSO or rapamycin (n = 3 biological replicates per group). *P = 0.0351, *P = 0.0131. ( f ) Quantification of expression levels of SASP genes ( Il1b , Il8 , Mmp3 and Ccl2 ) via RT‒qPCR in GCs isolated from 3-NP-treated WT and Ncoa7 − / − mice treated with DMSO or rapamycin (n = 4 biological replicates per group). ns P = 0.8979, ns P = 0.7816, ns P = 0.3738, ns P = 0.6572, *P = 0.0376, *P = 0.0321, **P = 0.0070, **P = 0.0045. ( g ) Quantification of the ovary index in 3-NP-treated WT and Ncoa7 − / − mice treated with DMSO or rapamycin (n = 8 biological replicates per group). ns P = 0.2740, ns P = 0.0695. Data in c - g are expressed as means ± s.e.m, and compared by two-tailed Student’s t-test.

Techniques: Western Blot, Staining, Mutagenesis, Immunofluorescence, Knockdown, Expressing, Isolation, Two Tailed Test

( a ) Schematic diagram showing the experimental design with rapamycin treatment in GCs from control, POI, and middle-aged participants. ( b ) Western blot of LC3 and p62 protein levels in GCs from control and POI women treated with DMSO or rapamycin. ( c ) Quantification of cell proliferation of GCs from POI and middle-aged participants treated with DMSO or rapamycin (n = 5 biological replicates per group). ****P < 0.0001, ****P < 0.0001, ****P < 0.0001, ****P < 0.0001. ( d ) Expression levels of SASP genes ( IL1B , IL8 , MMP3 and CCL2 ) via RT‒qPCR in GCs from control, POI, and middle-aged participants treated with DMSO or rapamycin (n = 4 biological replicates per group). ns P = 0.0995, ns P = 0.3605, ns P = 0.6335, ns P = 0.5805, *P = 0.0140, *P = 0.0285, **P = 0.0042, **P = 0.0083, **P = 0.0020, *P = 0.0205, *P = 0.0238, *P = 0.0120. ( e ) Schematic diagram showing the experimental design for N7 mRNA-LNP treatment in GCs from control, POI and middle-aged participants. ( f ) Western blot of NCOA7 protein levels in GCs from POI and middle-aged participants treated with scrambled mRNA-LNPs or N7 mRNA-LNPs. ( g ) Immunofluorescence of SGs in GCs from control, POI, and middle-aged participants treated with scrambled mRNA-LNPs or N7 mRNA-LNPs. Scale bar: 20 μm or 5 μm. ( h ) Quantification of cell proliferation of GCs from POI and middle-aged participants treated with scrambled mRNA-LNPs or N7 mRNA-LNPs (n = 5 biological replicates per group). ****P < 0.0001, ****P < 0.0001, *P = 0.0133, ****P < 0.0001. ( i ) Expression levels of SASP genes ( IL1B , IL8 , MMP8 , CCL2 ) in GCs from control, POI and middle-aged participants treated with scrambled mRNA-LNPs or N7 mRNA-LNPs (n = 4 biological replicates per group). ns P = 0.1461, ns P = 0.9336, ns P = 0.3977, ns P = 0.8452, ***P = 0.0005, **P = 0.0069, ***P = 0.0003, **P = 0.0069, **P = 0.0010, *P = 0.0120, ***P = 0.0005, **P = 0.0067. Data in c - d and h - i are expressed as means ± s.e.m, and compared by two-tailed Student’s t-test.

Journal: Nature Aging

Article Title: Stress granule clearance mediated by V-ATPase-interacting protein NCOA7 mitigates ovarian aging

doi: 10.1038/s43587-025-00927-w

Figure Lengend Snippet: ( a ) Schematic diagram showing the experimental design with rapamycin treatment in GCs from control, POI, and middle-aged participants. ( b ) Western blot of LC3 and p62 protein levels in GCs from control and POI women treated with DMSO or rapamycin. ( c ) Quantification of cell proliferation of GCs from POI and middle-aged participants treated with DMSO or rapamycin (n = 5 biological replicates per group). ****P < 0.0001, ****P < 0.0001, ****P < 0.0001, ****P < 0.0001. ( d ) Expression levels of SASP genes ( IL1B , IL8 , MMP3 and CCL2 ) via RT‒qPCR in GCs from control, POI, and middle-aged participants treated with DMSO or rapamycin (n = 4 biological replicates per group). ns P = 0.0995, ns P = 0.3605, ns P = 0.6335, ns P = 0.5805, *P = 0.0140, *P = 0.0285, **P = 0.0042, **P = 0.0083, **P = 0.0020, *P = 0.0205, *P = 0.0238, *P = 0.0120. ( e ) Schematic diagram showing the experimental design for N7 mRNA-LNP treatment in GCs from control, POI and middle-aged participants. ( f ) Western blot of NCOA7 protein levels in GCs from POI and middle-aged participants treated with scrambled mRNA-LNPs or N7 mRNA-LNPs. ( g ) Immunofluorescence of SGs in GCs from control, POI, and middle-aged participants treated with scrambled mRNA-LNPs or N7 mRNA-LNPs. Scale bar: 20 μm or 5 μm. ( h ) Quantification of cell proliferation of GCs from POI and middle-aged participants treated with scrambled mRNA-LNPs or N7 mRNA-LNPs (n = 5 biological replicates per group). ****P < 0.0001, ****P < 0.0001, *P = 0.0133, ****P < 0.0001. ( i ) Expression levels of SASP genes ( IL1B , IL8 , MMP8 , CCL2 ) in GCs from control, POI and middle-aged participants treated with scrambled mRNA-LNPs or N7 mRNA-LNPs (n = 4 biological replicates per group). ns P = 0.1461, ns P = 0.9336, ns P = 0.3977, ns P = 0.8452, ***P = 0.0005, **P = 0.0069, ***P = 0.0003, **P = 0.0069, **P = 0.0010, *P = 0.0120, ***P = 0.0005, **P = 0.0067. Data in c - d and h - i are expressed as means ± s.e.m, and compared by two-tailed Student’s t-test.

Techniques: Control, Western Blot, Expressing, Immunofluorescence, Two Tailed Test

Review

mmp3 (R&D Systems)

Structured Review

Images

1) Product Images from "A point-of-care test to measure tacrolimus directly from unprocessed whole blood"

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Enzyme-linked Immunosorbent Assay:

Clinical Proteomics:

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